proinflammatory cytokines in the embolic model of cerebral ischemia in rat.
نویسندگان
چکیده
increased levels of proinflammatory cytokines have been recorded after the onset of transient or permanent brain ischemia and are usually associated with exacerbation of ischemic injury. embolic stroke model is more relevant to the pathophysiological situation in such patients, because the majority of ischemic injuries in humans are induced by old thrombi that originate from the heart and carotid arteries. therefore, the aim of the present study was to investigate changes of inflammatory cytokines after embolic stroke. rats were subjected to embolic stroke, induced by a natural old clot which was injected in middle cerebral artery (mca), or sham stroke, which the same volume of saline was injected into the mca. at 48 h after stroke induction, the levels of 5 cytokines (il-1α and β, il-6, ifn-γ and tnf-α) were determined in 500 µg of total protein using the bio-plex rat cytokine array (biorad), according to the manufacturer’s instructions in ischemic and non- ischemic cortices. while stroke animals showed infarctions and neurological deficits, we did not observe any cerebral infarction and neurological deficits in sham-operated animals. the levels of il-1α (p=0.000) and -β (p =0.004), il-6 (p =0.008), tnf-α (p =0.000) and ifn-γ (p =0.044) were significantly increased compared to sham treated animals. the findings of the present study suggest that part of ischemic injury in the embolic stroke may be mediated through the increased levels of inflammatory cytokines.
منابع مشابه
Proinflammatory cytokines in the embolic model of cerebral ischemia in rat.
Increased levels of proinflammatory cytokines have been recorded after the onset of transient or permanent brain ischemia and are usually associated with exacerbation of ischemic injury. Embolic stroke model is more relevant to the pathophysiological situation in such patients, because the majority of ischemic injuries in humans are induced by old thrombi that originate from the heart and carot...
متن کاملNeuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia
Objective(s): Stroke poses a crucial risk for mortality and morbidity. Our study aimed to investigate the effect of p-coumaric acid on focal cerebral ischemia in rats. Material and Methods: Rats were randomly divided into four groups, namely Group I (control rats), Group II (ischemia rats), Group III (6 hr ischemia + p-coumaric acid rats) and Group IV (24 hr ischemia + p-coumaric acid rats). C...
متن کاملNeuroprotective Consequences of Postconditioning on Embolic Model of Cerebral Ischemia in Rat
متن کامل
neuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia
objective(s): stroke poses a crucial risk for mortality and morbidity. our study aimed to investigate the effect of p-coumaric acid on focal cerebral ischemia in rats. material and methods: rats were randomly divided into four groups, namely group i (control rats), group ii (ischemia rats), group iii (6 hr ischemia + p-coumaric acid rats) and group iv (24 hr ischemia + p-coumaric acid rats). ce...
متن کاملneuroprotective consequences of postconditioning on embolic model of cerebral ischemia in rat
0
متن کاملPropofol Protects Against Focal Cerebral Ischemia via Inhibition of Microglia-Mediated Proinflammatory Cytokines in a Rat Model of Experimental Stroke
Ischemic stroke induces microglial activation and release of proinflammatory cytokines, contributing to the expansion of brain injury and poor clinical outcome. Propofol has been shown to ameliorate neuronal injury in a number of experimental studies, but the precise mechanisms involved in its neuroprotective effects remain unclear. We tested the hypothesis that propofol confers neuroprotection...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۳، شماره ۲، صفحات ۱۲۵-۱۳۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023